Angiodrastic Chemokines Production by Colonic Cancer Cell Lines

Purpose: To study the production of angiodrastic chemokines by colonic cancer cell lines. Methods: A pro-angiogenic factor (VEGF), two angiogenic (CXCL8, CXCL6), and one angiostatic (CXCL4) chemokine were measured ELISA in supernatants colon lines HT-29 Caco-2. Cells cultured for 24 h presence serum from patients or healthy individuals. Results analyzed one-way ANOVA General Linear Model repeated measures. Results: Colonic epithelial cells are potent producers chemokines. Caco-2 produce all four under basal conditions after incubation with human serum. The secretion response, however, was completely different. more CXCL8 VEGF irrespective culture conditions, while seem unresponsive respect to CXCL6 CXCL4. Moreover, when incubated serum, contrary which CXCL4 same conditions. Conclusions: studied, but their responses not uniform similar differently regulated compared these